What are stem cells?
Stem cells can be isolated from various sources, including the umbilical cord, Wharton’s Jelly, and cord blood. Umbilical cord blood mononuclear cells are composed of HSCs (Hematopoietic Stem Cells). MSCs (Mesenchymal Stem cells). Mesenchymal stem cells are unique cells that can transform into specialized cells. For example, a stem cell could become a brain, bone, kidney, or skin cell. When introduced to parts of the body that are aging or have damaged cells, stem cells reproduce, replacing the old cells with new ones using the power of your body’s natural healing processes for tissue repair and restoration. This process provides long-lasting results that don’t depend on artificial substances or surgical intervention. Stem cells used in conjunction with growth factors are an effective therapy for reversing the signs of aging. As we age, our cells become old, senescent, and dysfunctional. Umbilical stem cells are new, more youthful, and more viable than our aging cells.
What causes the face to age?
As we age, we lose collagen, elastin, fat, hyaluronic acid, bone, and muscle. Collagen gives the skin its firmness. 1% of your collagen is lost per year beginning in your 20’s. By your 60’s, 80% of collagen is lost. In women, collagen loss accelerates around and immediately after menopause. Studies suggest that women lose about 30% of skin collagen in the first five years of menopause. With the loss of collagen, the skin loses its ability to retain elasticity from elastin and moisture from hyaluronic acid. Elastin gives skin elasticity and strength, allowing it to return to its original shape. Loss of facial fat causes facial volume loss. Bone loss causes changes in the outer layers of soft tissue and skin of the face. These changes can result in frown lines on the forehead, crow’s feet, sagging and hollow areas under the eyes and cheeks, deep folds and wrinkles around the mouth, a sagging jawline, age spots, discoloration, and loss of a youthful glow. These effects are more pronounced in some people than others. Factors such as overexposure to the sun, genetics, and tobacco use can accelerate skin aging.
What are the effects of stem cell rejuvenation treatments?
By boosting the healing power of your body, stem cell rejuvenation acts in several ways to improve the condition of your skin:
- Restoring elasticity
- Improving the tone and texture of the skin
- Tightening skin
- Firming underlying facial tissues
- Hydrating skin
- Boosting antioxidant levels
The results of stem cell rejuvenation treatment begin immediately following your treatment session, and your skin continues to improve over time as new cells replace old ones.
Facial Rejuvenation with Wharton’s Jelly
What is Wharton’s Jelly?
Wharton’s Jelly is a gelatinous substance from the umbilical cord. It contains cells that can transform into nearly every cell type in the body.1 In studies on wound healing, Wharton’s jelly was found to stimulate cells within the skin (dermis), which are responsible for producing elastic fibers, glycosaminoglycans (essential in skin hydration), and collagen (firmness of skin). These cells (fibroblasts) secrete growth factors that maintain skin structure.2 The MSCs (Multipotent Stem Cells in Wharton’s Jelly) have been demonstrated to strongly improve tissue damage in response to skin injury by increasing collagen, angiogenesis (blood supply to tissue), and enhancing the growth of the outer layer of skin (epidermis).3,4,5,6 Based on wound healing studies, it can be extrapolated that Wharton’s jelly can potentially improve hydration by locking in moisture and keeping skin supple and plump.
About the Product:
Nano PRP Jelly is potent and has 70 mg tissue weight per 3 ml vial. The initial number of stem cells doesn’t determine the product’s rejuvenating function. The stem cells in the product last only 5 days. The most critical component of the product – the mesenchymal stem cells, which generate type 1 collagen, type 3 collagen, and hyaluronic acid – the building blocks needed for skin repair/regeneration – continue their function long after the stem cells are gone.
Where is Wharton’s Jelly used?
Fat loss in the temples, sunken area (tear troughs) under the eyes, volume loss in the cheeks and chin, the lower 1/3 of the face and neck.
How many treatments are required, and how often?
One vial per area. Three treatments four to six weeks apart
Facial Rejuvenation with Exosomes
What are Exosomes?
Exosomes are umbilical cord blood-derived mesenchymal stem cells. Mesenchymal stem cells can transform into other cell types. They contain a high concentration of growth factors, which can increase collagen and elastin production and the skin’s epithelial layer (outer layer). Exosomes have been found to decrease the enzyme MMP1 expression. MMP1 is an enzyme that destroys collagen and elastin. Exosomes can promote type I and type III collagen expression during early wound healing.7 Recent studies have investigated the effects of human umbilical cord-derived exosomes on reducing the harmful consequences of UV exposure on the skin. Studies found that exosomes can reduce apoptosis (death of cells) and senescence (cell aging), increase collagen type I expression, and decrease matrix metalloproteinase (MMP1) expression in photo-aged (sun-damaged) skin cells.7,8,9 Exosomes improve skin texture, reduce the appearance of wrinkles, and restore a youthful glow by enhancing the skin’s natural healing processes.
About the Exosome Products:
Nano AER, a mesenchymal stem cell Wharton’s jelly-derived formulation, contains 50 billion exosomes. It is one of the few formulations injected into the skin to stimulate tropoelastin, the precursor of elastin, and GDF11. Growth differentiation factor 11 (GDF11) is a protein that regulates skin biology and has anti-aging effects on the skin. GD11 inhibits the inflammatory response, regenerates skin stem cells, regulates the production of procollagen 1 and hyaluronic acid, activates the Smad2/3 phosphorylation pathway in skin endothelial cells, and improves skin vasculature.7,8
How many treatments are required, and how often?
3 treatments 6 weeks apart
Topical Exosome Facial Microneedling
Microneedling with exosomes can be performed with Nano EX or Nano Xsomes. Nano EX contains 30 billion lyophilized exosomes with peptides, proteins, and growth factors essential for collagen-based healing. Nano Xsomes contain 180 billion Exosomes.
How many treatments are required, and how often?
3 treatments 6 weeks apart
Umbilical Stem Cells for Hair Restoration
What are umbilical stem cells, and how do they work?
Stem cells come from umbilical cord donations. They accelerate the initiation from the resting phase (telogen) of hair growth to the growth phase (anagen) of hair growth, increase the number of hairs, and increase the expression of proteins that trigger hair growth. Stem cells can regenerate the hair follicle bulb. Stem cell therapy potentially provides an alternative therapeutic method for the treatment of alopecia.
Why use umbilical stem cells?
Umbilical stem cells duplicate themselves every 22-28 hours for up to 65 generations. A person 50 years old or older’s bone marrow aspirate and adipose fat-derived stem cells multiply at a slower rate of 3 to 5 days for only 11 to 13 generations.
Are umbilical stem cells safe?
Umbilical stem cell products come from Umbilical Cord Blood donations from healthy babies delivered by C-section. The umbilical cell cord blood is obtained via a non-invasive, simple, and painless procedure after the baby is safely born. After prior authorization for donation is received (represented by the mother of the newborn child), an appropriately licensed establishment performs collection of the donor tissue in an aseptic manner. These donations are obtained under informed consent and according to strict ethical guidelines and protocols. Donor eligibility is carefully evaluated as required by the FDA by AATB standards and applicable state guidelines. Tissue donors are evaluated for high-risk behaviors. Screening includes reviewing the donor’s medical and social history, a physical assessment, serological screening for infectious disease, and tissue collection microbiology to ensure the umbilical stem cells are free of contagious disease.
How many umbilical stem cell treatments are required?
The number and frequency of treatments required depend on the area and condition to be treated.
Scalp Microneedling with Exosomes for Hair Restoration
Nano Ex
Scalp microneedling for hair restoration can be performed Nano EX contains 30 billion Lyophilized Exosomes with peptides, proteins, and growth factors, or Nano Xsomes contains 180 billion Exosomes
How often is the procedure performed?
2 weeks after the scalp microneedling treatment with Nano Ex or Nano Xsomes a treatment with Nano PRP Jelly can be done for improved results
Are Stem Cells Safe?
The cells are harvested aseptically – processed in a sterile manner, and distributed by our FDA-registered lab in accordance with Current Good Manufacturing Practices (cGMP), Current Good Tissue Practice (cGTP), state regulations, AATB guidelines, FDA requirements for Human Cells, Tissue, and Cellular and Tissue-based Products (HCT/P) in CFR Part 21, 1271, 361 and additional requirements for manufacturers of Human cells, Tissues, and Cellular and Tissue-based Products (HCT/Ps). A sample of each lot is tested by an independent AABB-compliant laboratory for any detectable infectious agents before release of the lot, as required.
Research has shown that stem cells are not susceptible to malignant transformation in a serum-free medium, making them safe for therapeutic use in patients.
However, these products are not FDA-approved for the aforementioned applications.
References:
1) Bakhtyar N, Jeschke MG, Herer E, Sheikholeslam M, Amini-Nik S. Exosomes from acellular Wharton’s jelly of the human umbilical cord promotes skin wound healing. Stem Cell Res Ther. 2018 Jul 13;9(1):193. doi: 10.1186/s13287-018-0921-2. PMID: 30005703; PMCID: PMC6044104.
2) Taissa Novis, Christina Maeda Takiya,Editor (s) Reginal Coeli dos Santos. Chapter 9 – Skin Resident Stem Cells and Regenerative Therapy (Second Edition), Academic Press, 2024 pages 205-249, ISBN 9780443152894
3) Rostamsadeh A, Anijomshoa M, Kurd S, Chai J, Jahangiri F, et al. The Role of Wharton’s Jelly Mesenchymal Stem Cells in Skin Reconstuction. J Skin Stem Cell. 2015;2(2)e60143
4) Fathke C, Wilson L, Hutter J, Kapoor V, Smith A, Hocking A, et al. Contribution of bone marrow-derived cells to skin: collagen deposition and wound repair. Stem Cells. 2004;22(5):812-22. [PubMed ID: 1534294]. . https://doi.org/10.17795/jssc30347 15342945.
5) Halkos ME, Zhao ZQ, Kerendi F, Wang NP, Jiang R, Schmarkey LS, et al. Intravenous infusion of mesenchymal stem cells enhances regional perfusion and improves ventricular function in a porcine model of myocardial infarction. Basic Res Cardiol. 2008;103(6):525-36. [PubMed ID: 18704259]. https://doi.org/10.1007/s00395-008-0741-0.
6) Nakagawa H, Akita S, Fukui M, Fujii T, Akino K. Human mesenchymal stem cells successfully improve skin-substitute wound healing. Br J Dermatol. 2005;153(1):29-36. [PubMed ID: 16029323]. https://doi.org/10.1111/j.1365-2133.2005.06554.x.
7) Hajialiasgary Najafabadi A, Soheilifar MH, Masoudi-Khoram N. Exosomes in skin photoaging: biological functions and therapeutic opportunity. Cell Commun Signal. 2024 Jan 12;22(1):32. doi: 10.1186/s12964-023-01451-3. PMID: 38217034; PMCID: PMC10785444.)
8) Liu S-J, Meng M-Y, Han S, Gao H, Zhao Y-Y, Yang Y, Lin Z-Y, Yang L-R, Zhu K, Han R. Umbilical cord mesenchymal stem cell-derived exosomes ameliorate HaCaT cell photo-aging. Rejuvenation Res. 2021;24:283–293. [PubMed] [Google Scholar
9) Zhang K, Yu L, Li F-R, Li X, Wang Z, Zou X, Zhang C, Lv K, Zhou B, Mitragotri S. Topical application of exosomes derived from human umbilical cord mesenchymal stem cells in combination with sponge spicules for treatment of photoaging. Int J Nanomedicine. 2020;15:2859–2872. [PMC free article] [PubMed] [Google Scholar]
10) Rochette L, Mazini L, Meloux A, Zeller M, Cottin Y, Vergely C, Malka G. Anti-Aging Effects of GDF11 on Skin. Int J Mol Sci. 2020 Apr 9;21(7):2598. doi: 10.3390/ijms21072598. PMID: 32283613; PMCID: PMC7177281.
11) Idkowiak-Baldys J, Santhanam U, Buchanan SM, Pfaff KL, Rubin LL, Lyga J. Growth differentiation factor 11 (GDF11) has pronounced effects on skin biology. PLoS One. 2019 Jun 10;14(6):e0218035. doi: 10.1371/journal.pone.0218035.
